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crystallization of human erythrocyte anion channel protein and circular dichroism spectroscopy of bacillus thuringiensis-insecticide and magainin-like antibiotics (endotoxins, toxins). [microform]
crystallization of human erythrocyte anion channel protein and circular dichroism spectroscopy of bacillus thuringiensis-insecticide and magainin-like antibiotics (endotoxins, toxins). [microform]
상세정보
- 자료유형
- 마이크로피시
- 청구기호
- 저자명
- 서명/저자
- crystallization of human erythrocyte anion channel protein and circular dichroism spectroscopy of bacillus thuringiensis-insecticide and magainin-like antibiotics (endotoxins, toxins). - [microform]
- 발행사항
- 형태사항
- 336 p. : microfiches ; 11×15 cm.
- 총서명
- UMI Dissertation
- 주기사항
- Source: Dissertation Abstracts International, Volume: 53-07, Section: B, page: 3462.
- 학위논문주기
- thesis (ph.d.)-- - rensselaer polytechnic institute, 1992.
- 초록/해제
- 요약The main goal of this thesis work is the structural studies of three types of biopolymers in order to elucidate the correlation between structure and function. Several physical and chemical methods were used to study the $\delta$-endotoxin from insecticide Bacillus thuringiensis (BT), the antibiotics magainin and PGLa, and human erythrocyte anion channel protein, also known as Band 3.
- 초록/해제
- 요약Delta-Endotoxin is a paracrystalline protein (protoxin) produced by BT. Toxins from different subspecies of BT kill different classes of insects. Subspecies Kurstaki used for the present studies, kills insect larvae belonging to the class Lepidopteran upon ingestion. The protoxin is cleaved to produce a toxin molecule in the gut of the insect. Circular dichroism (CD) spectroscopy and gel-electrophoresis (SDS-PAGE) were used to study the conformation of both the protoxin and the toxin molecules. It was found that both these proteins undergo conformational changes with varying pH and these changes may be correlated with pH-dependent change in the toxic activity of BT.
- 초록/해제
- 요약PGLa and magainin are 21-residue and 23-residue polypeptide antibiotics. CD spectroscopy was used to examine the conformation of these molecules in a variety of environments, as a basis for understanding their structural features. In addition, phospholipid binding studies were used to demonstrate features which permit the association of these polypeptides with membranes. These studies suggest that channels formed from a bundle of helices may be appropriate models for both of these antibiotics.
- 초록/해제
- 요약Band 3 is the 95 kDa integral membrane glycoprotein of the erythrocyte membrane. This protein is responsible for the exchange of chloride and bicarbonate across the erythrocyte cell membranes. Band 3 was isolated and purified from red blood cell membranes. Various techniques such as high pressure liquid size exclusion chromatography, SDS-PAGE and CD spectroscopy were used to characterize the purified protein. The protein was crystallized out of detergent solution. A number of morphologically distinct microcrystals (dimensions $\sim$0.3 x 0.05 x 0.2 mm) were prepared (Venugopal & Wallace, 1990). Preliminary diffraction studies indicate that while they are disordered, they do diffract to 3 A resolution.
- 복제주기
- Microfiche : UMI . microfiches;11×15 cm.
- 일반주제명
- 일반주제명
- 일반주제명
- 키워드
- 기타저자
- 기본자료저록
- Dissertation Abstracts International. 53-07B.
MARC
008970923s1992 us eng■001MOKWON00232997
■001AAV9236249
■00520010328155859
■008970923s1992 us eng
■035 ▼a(UnM)AAV9236249
■040 ▼aUnM▼cUnM▼dMOKWON
■090 ▼a540▼bV471c
■1001 ▼avenugopal, manju grover.
■24510▼acrystallization of human erythrocyte anion channel protein and circular dichroism spectroscopy of bacillus thuringiensis-insecticide and magainin-like antibiotics (endotoxins, toxins).▼h[microform]
■260 ▼aU.S.▼brensselaer polytechnic institute▼c1992.
■300 ▼a336 p.▼bmicrofiches▼c11×15 cm.
■350 ▼a$50.6
■44000▼aUMI Dissertation
■500 ▼aSource: Dissertation Abstracts International, Volume: 53-07, Section: B, page: 3462.
■502 ▼athesis (ph.d.)--▼brensselaer polytechnic institute▼d1992.
■520 ▼aThe main goal of this thesis work is the structural studies of three types of biopolymers in order to elucidate the correlation between structure and function. Several physical and chemical methods were used to study the $\delta$-endotoxin from insecticide Bacillus thuringiensis (BT), the antibiotics magainin and PGLa, and human erythrocyte anion channel protein, also known as Band 3.
■520 ▼aDelta-Endotoxin is a paracrystalline protein (protoxin) produced by BT. Toxins from different subspecies of BT kill different classes of insects. Subspecies Kurstaki used for the present studies, kills insect larvae belonging to the class Lepidopteran upon ingestion. The protoxin is cleaved to produce a toxin molecule in the gut of the insect. Circular dichroism (CD) spectroscopy and gel-electrophoresis (SDS-PAGE) were used to study the conformation of both the protoxin and the toxin molecules. It was found that both these proteins undergo conformational changes with varying pH and these changes may be correlated with pH-dependent change in the toxic activity of BT.
■520 ▼aPGLa and magainin are 21-residue and 23-residue polypeptide antibiotics. CD spectroscopy was used to examine the conformation of these molecules in a variety of environments, as a basis for understanding their structural features. In addition, phospholipid binding studies were used to demonstrate features which permit the association of these polypeptides with membranes. These studies suggest that channels formed from a bundle of helices may be appropriate models for both of these antibiotics.
■520 ▼aBand 3 is the 95 kDa integral membrane glycoprotein of the erythrocyte membrane. This protein is responsible for the exchange of chloride and bicarbonate across the erythrocyte cell membranes. Band 3 was isolated and purified from red blood cell membranes. Various techniques such as high pressure liquid size exclusion chromatography, SDS-PAGE and CD spectroscopy were used to characterize the purified protein. The protein was crystallized out of detergent solution. A number of morphologically distinct microcrystals (dimensions $\sim$0.3 x 0.05 x 0.2 mm) were prepared (Venugopal & Wallace, 1990). Preliminary diffraction studies indicate that while they are disordered, they do diffract to 3 A resolution.
■533 ▼aMicrofiche▼cUMI▼emicrofiches;11×15 cm.
■590 ▼aSchool code: 0185.
■650 4▼aChemistry, Biochemistry
■650 4▼aBiophysics, General
■650 4▼aChemistry, General
■653 ▼acrystallization▼aof▼ahuman▼aerythrocyte▼aanion▼achannel▼aprotein▼aand▼acircular▼adichroism▼aspectroscopy▼aof▼abacillus▼athuringiensis-insecticide▼aand▼amagainin-like▼aantibiotics▼a(endotoxins▼atoxins).
■690 ▼a0487
■690 ▼a0786
■690 ▼a0485
■71020▼arensselaer polytechnic institute.
■7730 ▼tDissertation Abstracts International▼g53-07B.
■790 ▼a0185
■791 ▼aPH.D.
■792 ▼a1992
