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isolation and characterization of branched meiotic recombination intermediates from both wildtype and mutant strains of the yeast saccharomyces cerevisiae. [microform]
isolation and characterization of branched meiotic recombination intermediates from both wildtype and mutant strains of the yeast saccharomyces cerevisiae. [microform]
상세정보
- 자료유형
- 마이크로피시
- 청구기호
- 저자명
- 서명/저자
- isolation and characterization of branched meiotic recombination intermediates from both wildtype and mutant strains of the yeast saccharomyces cerevisiae. - [microform]
- 발행사항
- 형태사항
- 214 p. : microfiches ; 11×15 cm.
- 총서명
- UMI Dissertation
- 주기사항
- Source: Dissertation Abstracts International, Volume: 57-10, Section: B, page: 6096.
- 학위논문주기
- thesis (ph.d.)-- - harvard university, 1996.
- 초록/해제
- 요약A branched DNA recombination intermediate (joint molecules, JM) has been isolated and physically characterized from meiotic cultures of the yeast Saccharomyces cerevisiae. JMs have the following characteristics: (1) Their occurrence is meiosis-specific, (2) Their abundance correlates with loci active in meiotic recombination and meiosis-specific DNA double strand break (DSB) formation, (3) They are formed after DSBs and prior to mature recombinant DNA duplexes, (4) Mutants that specifically block meiotic recombination coordinately block formation of JMs, (5) JMs contain full length DNA strands from bath parental homologs, (6) Approximately a quarter of these component strands are recombinant for an internal region, (7) JMs can be quantitatively resolved in vitro into both parental and recombinant duplexes using nucleases that specifically resolve Holliday junctions. From these observations, I conclude that JMs represent bona fide recombination intermediates. Further structural considerations indicate that JMs represent double Holliday junctions, an intermediate previously proposed in the repair of DSBs.
- 초록/해제
- 요약JMs form predominately between the two parental homologs, with only a small fraction forming between sister chromatids. To search for the genetic basis of this meiotic preference toward interhomolog recombination, JM formation was assayed in previously identified meiotic recombination mutants. Sequence homologs to the E. coli recA protein, and the meiotic chromatin structure protein RED1 were analyzed. These studies provide strong evidence that recombination between sister chromatids and homologs are differentially regulated, probably at a point prior to any physical change in the DNA. Meiosis-specific components RED1 and the meiosis-specific recA homolog DMC1 alter the normal mitotic preference for intersister recombination to the interhomolog mode favored during meiosis. The mitotic recA homologs RAD51, 55 and 57 function together during mitotic recombination, and are likely to function as the "core" of the recombination machinery.
- 복제주기
- Microfiche : UMI . microfiches;11×15 cm.
- 일반주제명
- 일반주제명
- 키워드
- 기타저자
- 기본자료저록
- Dissertation Abstracts International. 57-10B.
MARC
008970923s1996 us eng■001MOKWON00234250
■001AAV9710475
■00519990923093810
■008970923s1996 us eng
■035 ▼a(UnM)AAV9710475
■040 ▼aUnM▼cUnM▼dMOKWON
■090 ▼a574▼bS398i
■1001 ▼aschwacha, anthony.
■24510▼aisolation and characterization of branched meiotic recombination intermediates from both wildtype and mutant strains of the yeast saccharomyces cerevisiae.▼h[microform]
■260 ▼aU.S.▼bharvard university▼c1996.
■300 ▼a214 p.▼bmicrofiches▼c11×15 cm.
■350 ▼a$50.6
■44000▼aUMI Dissertation
■500 ▼aSource: Dissertation Abstracts International, Volume: 57-10, Section: B, page: 6096.
■502 ▼athesis (ph.d.)--▼bharvard university▼d1996.
■520 ▼aA branched DNA recombination intermediate (joint molecules, JM) has been isolated and physically characterized from meiotic cultures of the yeast Saccharomyces cerevisiae. JMs have the following characteristics: (1) Their occurrence is meiosis-specific, (2) Their abundance correlates with loci active in meiotic recombination and meiosis-specific DNA double strand break (DSB) formation, (3) They are formed after DSBs and prior to mature recombinant DNA duplexes, (4) Mutants that specifically block meiotic recombination coordinately block formation of JMs, (5) JMs contain full length DNA strands from bath parental homologs, (6) Approximately a quarter of these component strands are recombinant for an internal region, (7) JMs can be quantitatively resolved in vitro into both parental and recombinant duplexes using nucleases that specifically resolve Holliday junctions. From these observations, I conclude that JMs represent bona fide recombination intermediates. Further structural considerations indicate that JMs represent double Holliday junctions, an intermediate previously proposed in the repair of DSBs.
■520 ▼aJMs form predominately between the two parental homologs, with only a small fraction forming between sister chromatids. To search for the genetic basis of this meiotic preference toward interhomolog recombination, JM formation was assayed in previously identified meiotic recombination mutants. Sequence homologs to the E. coli recA protein, and the meiotic chromatin structure protein RED1 were analyzed. These studies provide strong evidence that recombination between sister chromatids and homologs are differentially regulated, probably at a point prior to any physical change in the DNA. Meiosis-specific components RED1 and the meiosis-specific recA homolog DMC1 alter the normal mitotic preference for intersister recombination to the interhomolog mode favored during meiosis. The mitotic recA homologs RAD51, 55 and 57 function together during mitotic recombination, and are likely to function as the "core" of the recombination machinery.
■533 ▼aMicrofiche▼cUMI▼emicrofiches;11×15 cm.
■590 ▼aSchool code: 0084.
■650 4▼aBiology, Molecular
■650 4▼aBiology, Genetics
■653 ▼aisolation▼aand▼acharacterization▼aof▼abranched▼ameiotic▼arecombination▼aintermediates▼afrom▼aboth▼awildtype▼aand▼amutant▼astrains▼aof▼athe▼ayeast▼asaccharomyces▼acerevisiae.
■690 ▼a0307
■690 ▼a0369
■71020▼aharvard university.
■7730 ▼tDissertation Abstracts International▼g57-10B.
■790 ▼a0084
■791 ▼aPH.D.
■792 ▼a1996
