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genetic and phenetic differences in two biotypes of the fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae). [microform]
genetic and phenetic differences in two biotypes of the fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae). [microform]
상세정보
- 자료유형
- 마이크로피시
- 청구기호
- 저자명
- 서명/저자
- genetic and phenetic differences in two biotypes of the fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae). - [microform]
- 발행사항
- 형태사항
- 119 p. : microfiches ; 11×15 cm.
- 총서명
- UMI Dissertation
- 주기사항
- Source: Dissertation Abstracts International, Volume: 57-11, Section: B, page: 6726.
- 학위논문주기
- thesis (ph.d.)-- - rutgers the state university of new jersey - new brunswick, 1996.
- 초록/해제
- 요약Spodoptera frugiperda is a migratory pest that infests corn, rice and pasture grasses throughout much of the Western Hemisphere. It consists of two morphologically indistinguishable biotypes, which may be host associated, the corn and rice strains. I designed these studies to: (1) compare the sequence divergence between the two strains of S. frugiperda and provide a diagnostic molecular genetic marker for distinguishing them; (2) document their seasonal occurrence and host use patterns in New Jersey, and; (3) examine the utility of a multivariate approach using morphological differences between them as a method of identification.
- 초록/해제
- 요약I sequenced the first 683 base pairs of the mitochondrial cytochrome-c oxidase subunit I gene (COI) for five individuals each of the corn and rice strains and 2 individuals of a closely related species, S. ornithogalli. I found a sequence divergence of 1.71% between the strains, which is within the range of divergences for COI found within single species and between closely related species of other Lepidoptera. A restriction enzyme analysis of COI resulted in differentiation between the two strains using Hin fI and SacI in combination. Both strains are present in New Jersey throughout the infestation period in the summer. They are both found in the corn habitat as larvae and adults in a ratio of approximately 60% corn to 40% rice strains. A multivariate discriminant analysis on adult morphology resulted in a discriminant function which correctly identified laboratory reared male corn strain moths 70% and rice strain moths 93% of the time. The same function used on field collected males, however, did not differentiate well between the strains, correctly identifying corn and rice strain males only 13% and 63% of the time, respectively. The results I present here indicate that there are quantifiable genetic differences between the two strains which can easily be used to distinguish the two mt-haplotypes. The lack of host association causes me to question the taxonomic status of the two strains as host-associated races in the process of sympatric speciation, while the morphological similarities between the two strains in the field supports the theory of reproductively isolated sibling species.
- 복제주기
- Microfiche : UMI . microfiches;11×15 cm.
- 일반주제명
- 일반주제명
- 일반주제명
- 키워드
- 기타저자
- 기본자료저록
- Dissertation Abstracts International. 57-11B.
MARC
008970923s1996 us eng■001MOKWON00234281
■001AAV9711092
■00519990928093150
■008970923s1996 us eng
■035 ▼a(UnM)AAV9711092
■040 ▼aUnM▼cUnM▼dMOKWON
■090 ▼a574▼bM111g
■1001 ▼amaas, paula ann young.
■24510▼agenetic and phenetic differences in two biotypes of the fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae).▼h[microform]
■260 ▼aU.S.▼brutgers the state university of new jersey - new brunswick▼c1996.
■300 ▼a119 p.▼bmicrofiches▼c11×15 cm.
■350 ▼a$50.6
■44000▼aUMI Dissertation
■500 ▼aSource: Dissertation Abstracts International, Volume: 57-11, Section: B, page: 6726.
■502 ▼athesis (ph.d.)--▼brutgers the state university of new jersey - new brunswick▼d1996.
■520 ▼aSpodoptera frugiperda is a migratory pest that infests corn, rice and pasture grasses throughout much of the Western Hemisphere. It consists of two morphologically indistinguishable biotypes, which may be host associated, the corn and rice strains. I designed these studies to: (1) compare the sequence divergence between the two strains of S. frugiperda and provide a diagnostic molecular genetic marker for distinguishing them; (2) document their seasonal occurrence and host use patterns in New Jersey, and; (3) examine the utility of a multivariate approach using morphological differences between them as a method of identification.
■520 ▼aI sequenced the first 683 base pairs of the mitochondrial cytochrome-c oxidase subunit I gene (COI) for five individuals each of the corn and rice strains and 2 individuals of a closely related species, S. ornithogalli. I found a sequence divergence of 1.71% between the strains, which is within the range of divergences for COI found within single species and between closely related species of other Lepidoptera. A restriction enzyme analysis of COI resulted in differentiation between the two strains using Hin fI and SacI in combination. Both strains are present in New Jersey throughout the infestation period in the summer. They are both found in the corn habitat as larvae and adults in a ratio of approximately 60% corn to 40% rice strains. A multivariate discriminant analysis on adult morphology resulted in a discriminant function which correctly identified laboratory reared male corn strain moths 70% and rice strain moths 93% of the time. The same function used on field collected males, however, did not differentiate well between the strains, correctly identifying corn and rice strain males only 13% and 63% of the time, respectively. The results I present here indicate that there are quantifiable genetic differences between the two strains which can easily be used to distinguish the two mt-haplotypes. The lack of host association causes me to question the taxonomic status of the two strains as host-associated races in the process of sympatric speciation, while the morphological similarities between the two strains in the field supports the theory of reproductively isolated sibling species.
■533 ▼aMicrofiche▼cUMI▼emicrofiches;11×15 cm.
■590 ▼aSchool code: 0190.
■650 4▼aBiology, Entomology
■650 4▼aBiology, Genetics
■650 4▼aBiology, Ecology
■653 ▼agenetic▼aand▼aphenetic▼adifferences▼ain▼atwo▼abiotypes▼aof▼athe▼afall▼aarmyworm▼aspodoptera▼afrugiperda▼a(j.▼ae.▼asmith)▼a(lepidoptera▼anoctuidae).
■690 ▼a0353
■690 ▼a0369
■690 ▼a0329
■71020▼arutgers the state university of new jersey - new brunswick.
■7730 ▼tDissertation Abstracts International▼g57-11B.
■790 ▼a0190
■791 ▼aPH.D.
■792 ▼a1996
